CBC 18th Annual Symposium: POSTER SESSION
Chicago Biomedical Consortium (CBC)
Thank you for joining us at the CBC 18th Annual Symposium: "Immunology and Immunoengineering Response to COVID-19" and welcome to the CBC Virtual Poster Session!
Feel free to browse through the posters during the Poster Session between 12:10 PM - 12:50 PM. Some posters may include links to pre-recorded presentations and some may include a "Chat with Presenter" button where, if clicked, will take you to a zoom session where you can chat with the presenter and ask questions. We welcome any comments. Just click on the "Join the Discussion" button and leave a message for the poster owner!
Posters will remain online and accessible over the weekend between 11/5 - 11/7. You will be able to leave comments and watch any pre-recorded videos, but the "Chat with Presenter" button will no longer be available.
Thank you again! Enjoy and don't forget to log back into the symposium webinar at 12:50 PM using this link: https://northwestern.zoom.us/j/92227344054
More info: https://chicagobiomedicalconsortium.org/education/symposia/symposia-2021/
Alzheimer’s Disease Related Neuroimmunological Consequences of COVID-19 in vaccinated Older African Americans
Diana Grass - Rutgers University Newark
EPITHELIAL GROWTH FACTOR TRANSCRIPTS IDENTIFIED IN CROATALUS ATROX VENOM FOR MEDICAL PROPERTIES AND FUNCTIONS
Ivan Lopez, Ying Jia Biology Department, The University of Texas Rio Grande Valley
A SUBSTANTIAL AND PERSISTENT HUMORAL RESPONSE TO THE PFIZER BNTB162B2 VACCINE DEVELOPS IN PATIENTS UNDERGOING MAINTENANCE HEMODIALYSIS
Yi-Shin Chang, UIC; Kai Huang, UIC; Christen L Vagts, UIC; Christian Ascoli, UIC; Md-Ruhul Amin, UIC; Mahmood Ghassemi, UIC; Claudia M Lora, UIC; Russell Edafetanure-Ibeh, UIC; Yue Huang, UIC; Ruth A Cherian, UIC; Nandini Sarup, UIC; Samantha R Warpecha, UIC; Sunghyun Hwang, UIC; Rhea Goel, UIC; Benjamin A Turturice, UIC, Stanford U; Cody Schott, UIC, UCDenver; Montserrat Hernandez, UIC; Yang Chen, UIC; Julianne Jorgensen, UIC; Wangfei Wang, UIC; Mladen Rasic, UIC; Richard M Novak, UIC; Patricia W Finn, UIC; David L Perkins, UIC.
Methods: The humoral immune response to the Covid-19 BNT162b2 mRNA vaccine was assessed in 20 HD patients and cohort-matched controls. Anti-Spike IgG titers and antibody neutralization assays were quantified prior to the first vaccination dose (V1D0) and seven days after the second dose (V2D7). Anti-Spike IgG titers were additionally quantified six months after initial vaccination. Clinical history and lab values in HD patients were obtained to identify baseline and early predictors of vaccination response.
Results: Anti-Spike IgG titers and neutralizing function were substantially elevated in both controls and HD at V2D7, with only a slight reduction in titers in HD groups (p < 0.05). Anti-Spike IgG remained elevated above baseline at six months in both subject groups. Anti-Spike IgG titers at V2D7 were highly predictive of 6-month titer levels. Additionally, baseline serum ferritin values in the intermediate range and log-fold change in WBCs were predictive of antibody development.
Conclusion: We show that patients on maintenance HD develop a substantial humoral immune response to the BNT162b2 mRNA COVID-19 vaccine, and that antibodies to the SARS-CoV-2 spike protein are persistently elevated six months after initial vaccination.
CBC Entrepreneurial Fellows Award Program
Michelle Hoffmann, CBC Executive Director
The Role of Epigenetic Changes in Pancreatic Beta Cells in the Etiology of Diabetes
Thomas Heinbockel, Antonei B. Csoka Department of Anatomy, Howard University, Washington DC, 20059
CBC Accelerator Award Program and CBCAN
The CBC Accelerator Network (CBCAN) program brings together industry experts, university tech transfer officers, researchers and others from the local and extended biomedical ecosystem with discoveries that may have commercial potential. The aim is to move promising discoveries into and forward in the pipeline towards commercialization, providing early commercial guidance that universities and university-based researchers need.
Sex Differences in Lung Inflammation in Asthmatic Mice Exposed to Ambient Ozone
Keishla Colón Montañez1 (UChicago) Fuentes, PhD1 and Patricia Silveyra, PhD1 (Indiana University Bloomington)
HYPERCAPNIA INCREASES ACE2 PROTEIN EXPRESSION AND PSEUDO-SARS-COV-2 VIRUS ENTRY IN EPITHELIAL CELLS THROUGH A STEROL-REGULATORY ELEMENT BINDING PROTEIN 2 (SREBP2)-DEPENDENT MECHANISM
S.Marina Casalino-Matsuda, NU; Fei Chen, NU; Aiko Matsuda, NU; Scott Budinger, NU; and Peter H.S. Sporn, NU
Methods: Differentiated HBE cells, BEAS-2B or VERO cells were incubated in normocapnia (5% CO2, PCO2 36 mmHg) or hypercapnia (15% CO2, PCO2 108 mmHg). ACE2 protein expression was assessed by immunoblot or immunofluorescence, and cholesterol by Amplex red assay. Also, cells pre-exposed to normocapnia or hypercapnia were infected with Pseudo SARS-CoV-2. For in vivo studies, C57BL/6 mice were pre-exposed to normoxic hypercapnia (10% CO2/21% O2) for 7 days, or air, and ACE2 expression in airways was assessed by immunofluorescence. SREBP2 was blocked using betulin or AM580.
Results: Hypercapnia increased cellular cholesterol and ACE2 protein expression in epithelial cells in vivo and in vitro. This effect was reversed by blocking SREBP2. Additionally, hypercapnia augmented Pseudo SARS-CoV-2 entry into cells, effect reduced by SREBP2 inhibitors.
Conclusion: Hypercapnia creates a high-cholesterol cellular environment that induces increased ACE2 expression and Pseudo-SARS-CoV-2 entry. This may lead to a greater burden of SARS-CoV-2 infection in patients with hypercapnia, and in part account for worse clinical outcomes of COVID-19 pneumonia in advanced COPD and other severe lung diseases.